ISOCITRATE LYASE AND MALATE SYNTHASE IN PSEUDOMONAS INDIGOFERA II
نویسندگان
چکیده
منابع مشابه
Isocitrate Lyase and Malate Synthase in Hydrogenomonas facilis*
Studies of assimilation of CY402 by a facultative chemoautotroph, Hydrogenomonas facilis, established that products similar to those for photosynthetic fixation were formed (1, 2). The observation that glutamic acid was more rapidly labeled than glycine (2) was consistent with the hypothesis first advanced by Smith and Gunsalus (3) that a key step in glycine synthesis by some microorganisms may...
متن کاملIsocitrate lyase and malate synthase genes from Brassica napus L. are active in pollen.
To investigate if pollen possesses glyoxysomal function, we analyzed the activities of isocitrate lyase and malate synthase genes. Because the activities of these enzymes were exceedingly low in pollen extracts, we constructed fusion genes encoding j3-glucuronidase (CUS) that are regulated by isocitrate lyase or malate synthase promoters from Brassica napus L. to increase the sensitivity of our...
متن کاملCoordinate expression of transcriptionally regulated isocitrate lyase and malate synthase genes in Brassica napus L.
We have analyzed the temporal and spatial expression of genes encoding the glycoxylate cycle enzymes isocitrate lyase and malate synthase in Brassica napus L. to determine whether they are coordinately expressed. Both enzymes participate in reactions associated with lipid mobilization in oilseed plant seedlings and are sequestered in a specialized organelle, the glyoxysome. We have identified a...
متن کاملMutants of Bacillus stearothermophilus de-repressed for isocitrate lyase and malate synthase.
philes Pseudomonas indigofera (Shiio et al., 1965) and Chlorella pyrenoidosa (John 8c Syrett, 1967) is apparently similar. In mutant PC2 NG35 cells the lyase accounts for 16 % of the soluble proteins. The corresponding proportion of malate synthase is 2.5% in the wild-type and 6% in the mutant. Themolecularweightsof thenativeenzymes, determined by gelfiltrationonaSepharose 6B column, are given ...
متن کاملIsocitrate lyase from Phycomyces blakesleeanus
Isocitrate lyase was purified from Phycomyces blakesleeanus N.R.R.L. 1555(-). The native enzyme has an Mr of 240000. The enzyme appeared to be a tetramer with apparently identical subunits of Mr 62000. The enzyme requires Mg2+ for activity, and the data suggest that the Mg2+-isocitrate complex is the true substrate and that Mg2+ ions act as a nonessential activator. The kinetic mechanism of the...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1962
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.84.6.1222-1227.1962